Newcastle disease virus(NDV) antibody ELISA for poultrykit
- Sep 09, 2026 Anon
Newcastle disease virus(NDV) antibody ELISA kit
Catalog No. LSY-30013
1. Brief
This kit is used to detect Newcastle disease virus(NDV) antibody in serum of poultry, to assess antibody condition by Newcastle disease virus(NDV) vaccine poultry and assist diagnosis of serological infected poultry.
This kit use blocking ELISA method, it can detect Newcastle disease antibodies in the serum of chickens, ducks, pigeons and other poultry. NDV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is NDV specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labeled anti-NDV virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelength to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
2. Reagents
Code | Item | Spec. | Code | Item | Spec. |
1 | NDV Ag Coated plates 96 wells | 1/2 plate | 6 | Stop solution | 15 ml |
2 | Enzyme Conjugate | 11/22 ml | 7 | Negative control | 2 ml |
3 | 10XConcentrated Washing buffer | 100 ml | 8 | Positive control | 1 ml |
4 | Substrate | 11/22 ml | 9 | Adhesive Foil | 2/4 pieces |
5 | Sample dilution | 100 ml | 10 | Instruction | 1 piece |
3. Materials required but not provided
1) Micropipette: 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells with 450/630nm wavelength.
5) Distilled water or deionized water.
6) Bottle washer or Microplate Washer
4. Sample preparation
Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.
5. Results
Read the OD value with microplate-reader at 450nm (630nm as reference).
For the test to be valid:
OD value of Negative control (N) >0.4;
Calculation method:
OD value of samples/ Average OD value of Negative control = S/N value
Result judge:
S/N ≥ 0.5, Negative;
S/N < 0.5, Positive.
Specifications: 96 or 96*2 wells/kit.
Expiry date: 12 months.
Storage: Storing at 2-8℃, in the dark.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
