Avian Infectious Bursal Disease virus VP2 antibody (IBDV-VP2 Ab) ELISA kit for chicken
- Sep 09, 2026 Anon
Avian Infectious Bursal Disease virus VP2 antibody
(IBDV-VP2 Ab) ELISA kit
Catalog No. LSY-30014
1. Introduction
This kit is used to detect Avian Infectious Bursal Disease virus Neutralizing antibody in chicken serum, to assess antibody condition by Avian Infectious Bursal Disease virus VP2 vaccine in chicken farm and assist diagnosis of serological infected chicken.
Chicken infectious bursal disease virus VP2 protein has a conformational (discontinuous) neutralizing antigenic determinant. Studies have found that antibodies against this antigenic determinant can passively protect chickens from infectious bursal disease virus infection.This kit use competitive ELISA method, composed by the reaction Micro-plate coated with high purity IBDV-VP2 antigen, horseradish peroxidase-labeled anti-chicken IgG and other reagents. The reaction mechanism is the coated antigen binding with IBDV-VP2-Ab in sample, and then with the enzyme-labeled anti-chicken IgG antibody to form a "coated antigen + IBDV-VP2-Ab + anti-chicken IgG HRP antibody" complex, add substrate, it will have coloration by the enzyme catalytic reaction. Color depth is proportional to the amount of IBDV-VP2-Ab, when the sample chromogenic reaction, the results detected by the microplate reader exceeds a set threshold value result judged as positive, indicating that the immune produced antibodies or natural infection exists.
2. The kit components
1 | IBD antigen coated microplate | 2 plates of 96 wells | 7 | Substrate B solution | 12 ml |
2 | Enzyme conjugate | 24 ml | 8 | Stop solution | 12 ml |
3 | 101X Concentrated VP2 Monoclonal antibody | 210 uL | 9 | Negative control | 100 uL |
4 | Monoclonal dilution | 24 ml | 10 | Positive control | 100 uL |
5 | 10×concentrated washing buffer | 100 ml | 11 | Adhesive film | 4 pieces |
6 | Substrate A solution | 12 ml | 12 | Instruction | 1 piece |
3. Materials Required But Not Provided
1) Micropipettors and disposable tips: 0.5μL~10μL、10μL~100μL、100μL~1000μL、1mL-10mL
2) Disposable tips
3) 37 ℃ Incubator
4) Measuring cylinder: 500 ml
5) 96 wells microplate reader
6) Distilled water or deionied water
7) Bottle or microplate washing machine
4. Sample preparation
Take animal whole blood, make serum according to regular methods, the serum should be clear, have no hemolysis.
5. Results
Set zero for the blank well, and test the A450nm (630 nm as a reference) value on the microplate-reader.
For the test to be valid:
OD value of Negative control (N) >1.0;
OD value of Positive control (P)<0.7.
If the test is invalid, the operation is in doubt, retest and observe all the reagents carefully.
Calculation method:
OD value of samples/ Average OD value of Negative control = S/N value
Result judge:
S/N ≥ 0.7, Negative;
S/N＜0.7, Positive.
Specifications: 96*2 wells/kit.
Expiry date: 12 months.
Storage: Storing at 2-8℃, in the dark.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
