Infectious Bronchitis Virus (IBV) antibody ELISA kit for poultry
- Sep 09, 2026 Anon
Infectious Bronchitis Virus (IBV) antibody ELISA kit
Catalog No. LSY-30017
1. Usage
This kit is used to detect Infectious Bronchitis Virus (IBV) antibody in chicken serum, to assess antibody condition by Infectious Bronchitis vaccine in chicken farm and assist diagnosis of serological infected chicken.
2. Principle
The Infectious Bronchitis Virus (IBV) antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).
3. Reagents
1 | IBV antigen coated microplate | 2 plates of 96 wells | 7 | Stop solution | 12 ml |
2 | Enzyme conjugate | 24 ml | 8 | Negative control | 1.5ml |
3 | 10×concentrated washing buffer | 100 ml | 9 | Positive control | 1.5ml |
4 | Substrate A solution | 12 ml | 10 | Serum dilution plate | 2 piece |
5 | Substrate B solution | 12 ml | 11 | Adhesive film | 4 pieces |
6 | Sample dilution | 100 ml | 12 | Instruction | 1 piece |
4. Materials required but not provided
1) Micropipettors and disposable tips: 0.5μL~10μL、10μL~100μL、100μL~1000μL
2) 37 ℃ Incubator
3) Measuring cylinder: 500 ml
4) 96 wells microplate reader
5) Distilled water or deionied water
6) Bottle or microplate washing machine
5. Results
Set zero for the blank well, and test the A450nm (630 nm as a reference) value on the microplate-reader. The conditions for the test to be tenable are that the positive control wells’ A450nm value is greater than or equal to 0.5, and the negative control wells’ A450nm value is less than 0.15. If the test is invalid, the operation is in doubt, retest and observe all the reagents carefully.
If the sample’s A450 value is greater than 0.15+ absorbance of negative control mean, it is judged to be positive; and if less than 0.15+ absorbance of negative control mean, negative. If absorbance of negative control mean is less than 0.05, calculate as 0.05
Specifications: 96 wells/kit or 96*2 wells/kit
Expiry date: 12 months.
Storage: Storing at 2-8℃, in the dark.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
