Mycoplasma Gallisepticum(MG) Antibody ELISA Test Kit For Poultry
- Sep 09, 2026 Anon
Mycoplasma Gallisepticum(MG) Antibody ELISA Test Kit
Catalog No. LSY-30026
1. Usage
This kit is used to detect Mycoplasma Gallisepticum(MG) antibody in chicken serum, to assess antibody condition by Mycoplasma Gallisepticum(MG) vaccine in chicken farm and assist diagnosis of serological infected chicken.
2. Principle
The Mycoplasma Gallisepticum(MG) antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).
3. Reagents
1 | MG antigen coated microplate | 1 plate of 96 wells | 7 | Stopping solution | 6ml |
2 | Enzyme conjugate solution | 12 ml | 8 | Negative control | 0.8ml |
3 | 10×concentrated washing buffer | 50ml | 9 | Positive control | 0.8ml |
4 | Substrate A solution | 6ml | 10 | Serum dilution plate | 1 piece |
5 | Substrate B solution | 6ml | 11 | Adhesive film | 2 pieces |
6 | Sample dilution | 50ml | 12 | Instruction | 1 piece |
4. Materials required but not provided
1) Micropipettors and disposable tips: 0.5μL~10μL、10μL~100μL、100μL~1000μL
2) Disposable tips
3) 37 ℃ Incubator
4) Measuring cylinder: 500 ml
5) 96 wells microplate reader
6) Distilled water or deionied water
7) Bottle or microplate washing machine
5. Results
Set zero at the blank control well, and test the OD450nm (630 nm as reference) value on the microplate-reader. The conditions for the test to be tenable are that the positive control wells’ average OD450nm value is greater than or equal to 0.4, and the negative control wells’ average OD450nm value is less than 0.15. If the test is invalid, the operation procedure is skeptical, run the test again and observe all the reagents carefully.
If the sample’s A450 value is greater than 0.25+ absorbance of negative control, it is judged to be positive; and if less than 0.25+ absorbance of negative control, negative. If absorbance of negative control is less than 0.05, calculate as 0.05
Specifications: 96 wells/kit.
Expiry date: 12 months.
Storage: Storing at 2-8℃, in the dark.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
