Pullorum Disease (PD) and Fowl Typhoid (FT) Antibody ELISA kit for chicken
- Sep 09, 2026 Anon
Pullorum Disease (PD) and Fowl Typhoid (FT) Antibody ELISA kit
Catalog No. LSY-30027
1. Usage
This kit is used to detect Pullorum Disease (PD) and Fowl Typhoid (FT) antibody in chicken serum, to assess antibody condition by Pullorum Disease (PD) and Fowl Typhoid (FT) vaccine in chicken farm and assist diagnosis of serological infected chicken.
2. Principle
The Pullorum Disease (PD) and Fowl Typhoid (FT) antibody ELISA kit is based on an indirect enzymatic immunoassay (Indirect ELISA).The antigen is coated on plates. When a sample serum contains specific antibodies against virus, they will bind to the antigen on plates. Wash the unbound antibodies and other components. Then add a specific enzyme conjugate. After incubation and washing, add the TMB substrate. A colorimetric reaction will appear, measured by a spectrophotometer (450 nm).
3. Reagents
1 | PD&FT Antigen coated microplate | 1/2 pieces | 7 | Negative control serum | 2 mL |
2 | Enzyme Conjugate | 11/22 mL | 8 | Positive control serum | 1.6 mL |
3 | 10×concentrated washing buffer | 100 mL | 9 | Serum diluent plate | 1/2 pieces |
4 | Substrate solution | 11/22 mL | 10 | Adhesive film | 2/4 pieces |
5 | Sample diluent | 100 mL | 11 | Instruction | 1 piece |
6 | Stop solution | 15 mL | 12 |
4. Materials required but not provided
1) Micropipettors and disposable tips: 0.5μL~10μL、10μL~100μL、100μL~1000μL
2) 37 ℃ Incubator
3) Measuring cylinder: 500 ml
4) 96 wells microplate reader
5) Distilled water or deionied water
6) Bottle or microplate washing machine
5. Results
Read the OD value by microplate-reader at wavelength of OD450nm (630 nm as reference).
The test to be valid :
OD value of Negative control (N)<0.2, meanwhile OD value of Positive control >0.4
Calculation method:
OD value of Sample/Average OD value of Positive Control=S/P value
Result judgement:
S/P<0.35: Negative;
S/P≥0.35: Positive.
Specifications: 96*2 wells/kit.
Expiry date: 12 months.
Storage: Storing at 2-8℃, in the dark.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
