Rabies virus antibody ELISA kit for dog
- Sep 09, 2026 Anon
Rabies virus antibody ELISA kit
Catalog No. LSY-30012
1. Introduction
The Rabies Virus (RBV) antibody ELISA kit is used to test rabies virus antibody in serum of dogs, cats, pigs, bovine and goat etc. It is used to evaluate the immune status of rabies virus vaccine or the infection of non immunized animals. This product is only used for veterinary diagnosis reference, not for human clinical diagnosis.
This kit use indirect ELISA method, rabies antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is rabies virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labeled anti-rabies virus antibody, antibodies in sample combine with the enzyme labeled anti-rabies virus antibodies; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelength to measure the OD value in reaction wells after adding stop solution to stop the reaction.
2. Reagents and contents
Code | Components | Specifications & Quantity |
96T | ||
1 | Rabies-Ag Coated plates 96wells | 1 plate |
2 | Enzyme Conjugate | 11ml *1 |
3 | 10X Concentrated washing buffer | 100ml *1 |
4 | Substrate | 11ml *1 |
5 | Sample diluent | 100ml *1 |
6 | Stop solution | 15ml *1 |
7 | Negative control | 2ml*1 |
8 | Positive control | 1.6ml*1 |
9 | Serum dilution plate | 1 piece |
10 | Adhesive Foil | 2 pieces |
11 | Instruction sheet | 1 piece |
3. Material required not provided
1) Micropipette: 0.5ul-10ul,10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells.
5) Distilled water or deionized water.
6) Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis
5. Storage and expire date
Store at 2~8℃ in dark, no frozen, expiry date: 12 months.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
