ELISA Echinococcosis Dog Feces Antigen kit For Dog
- Sep 09, 2026 Anon
ELISA Echinococcosis Dog Feces Antigen kit
Catalog No. LSY-30024
1. Usage
This test kit is used to detect Echinococcosis antigen in dog feces, used to help diagnosis for infection of Echinococcus granulosus in dogs and carnivores.
This test kit use double-antibody sandwich method, monoclonal antibody against Echinococcus granulosus is precoated on microplate. When run the test, add sample, then incubate, if there is Echinococcosis antigen in the sample, it will combine with the pre-coated monoclonal antibody; discard the uncombined antigen after washing, then add enzyme conjugate, forming pre-coated monoclonal antibody-antigen combination forming monoclonal antibody-enzyme conjugate complex; discard the uncombined enzyme conjugate; add substrate, the blue signal formed by the enzyme is proportional to the antigen content in the sample, measure the OD value by ELISA reader with wavelength at 450nm.
2. Reagents and contents
Code | Item | Spec. | Code | Item | Spec. |
1 | Echinococcus monoclonal antibody Coated plate 96wells | 2 plates | 6 | Stop solution | 15 ml |
2 | Enzyme Conjugate | 11 ml x2 | 7 | Negative control | 2 ml |
3 | 10X Concentrated washing buffer | 100 ml | 8 | Positive control | 2 ml |
4 | Substrate | 22 ml | 9 | Adhesive Foil | 4 pieces |
5 | 5X Sample treatment solution | 100 ml | 10 | Instruction sheet | 1 piece |
3. Material required not provided
1) Micropipette: 0.5ul-10ul, 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells.
5) Distilled water or deionized water.
6) Microplate Washer
4. Washing buffer and Sample treatment solution preparation
1) Return 10X Concentrated washing buffer into room temperature before use, if there is salt crystals, shake to make it dissolved, then dilute it at 10 times with distilled water or deionized water. The diluted washing buffer can store at 4℃ for about 1 week.
2) Return 5X Sample treatment solution into room temperature before use, if there is salt crystals, shake to make it dissolved, then dilute it at 5 times with distilled water or deionized water. The diluted Sample treatment solution can store at 4℃ for about 1 week.
5. Storage and expire date
Store at 2~8℃ in dark, no frozen, expiry date: 12 months.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
