Foot-and-mouth disease virus (FMDV) Type A Antibody ELISA Test Kit For Cattle and Sheep
- Sep 09, 2026 Anon
Foot-and-mouth disease virus (FMDV) Type A Antibody ELISA Test Kit
For Cattle and Sheep
Catalog No. LSY-30034
1. Principle
This kit is used to detect specific antibody against foot and mouth disease virus (FMDV) Type A Antibody in serum of cattle and sheep qualitatively, for monitoring antibody after FMDV Type A immune.
This kit use indirect ELISA method, pured FMDV antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is FMD virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-FMD virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelength to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
2. Reagents and contents
Code | Item | Spec. | Code | Item | Spec. |
1 | Antigen Coated plates 96 wells | 2 plates | 7 | Positive control | 1.6 ml |
2 | Enzyme Conjugate | 11 ml | 8 | Negative control | 1.6 ml |
3 | 10XConcentrated Washing buffer | 100 ml | 9 | Serum dilute plate | 2 plates |
4 | Substrate | 22 ml | 10 | Adhesive Foil | 4 pieces |
5 | Sample diluent | 100 ml | 11 | Instruction | 1 piece |
6 | Stop solution | 11 ml |
3. Material required not provided
1) Micropipette: 0.5-10ul, 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells with 450/630nm wavelenth.
5) Distilled water or deionized water.
6) Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis
5. Storage and expire date
Store at 2~8℃ in dark, no frozen, expiry date: 12 months.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
