Foot-and-mouth disease virus (FMDV) Non-structural protein Antibody 3ABC ELISA Test Kit
- Sep 09, 2026 Anon
Foot-and-mouth disease virus (FMDV) Non-structural protein
Antibody 3ABC ELISA Test Kit
Catalog No. LSY-30040
1. Principle
Foot-and-mouth disease virus (FMDV) Non-structural protein Antibody 3ABC ELISA Test Kit is suitable for test serum of cattle, sheep, goats and pigs, it can distinguish between immunized animals and wild-infected animals.
This kit use blocking ELISA method, FMDV-3ABC antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is FMD virus specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-FMD virus monoclonal antibody, antibody in sample block the combination of monoclonal antibody and pre-coated antigen; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelength to measure the absorbance OD value in reaction wells after adding stop solution to stop the reaction.
2. Reagents and contents
Code | Components | Specifications & Quantity |
192T | ||
1 | FMDV-3ABC Ag Coated plates 96 wells | 2 plates |
2 | Enzyme Conjugate | 11ml *2 |
3 | 10X Concentrated washing buffer | 100ml *1 |
4 | Substrate | 22ml *1 |
5 | Sample diluent | 30ml *1 |
6 | Stop solution | 15ml *1 |
7 | Negative control | 2ml *1 |
8 | Positive control | 1ml *1 |
9 | Adhesive Foil | 4 pieces |
10 | Instructions | 1 piece |
3. Material required not provided
1) Micropipette: 0.5μl~10μl、10μl~100μl、100μl~1000μl.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells with 450/630nm wavelength.
5) Distilled water or deionized water.
6) Bottle washer or Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis
5. Storage and expire date
Store at 2~8℃ in dark, no frozen, expiry date: 12 months.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
