Peste des petits ruminants virus (PPRV) antibody ELISA kit for cattle
- Sep 09, 2026 Anon
Peste des petits ruminants virus (PPRV) antibody ELISA kit
Catalog No. LSY-30035
1. Usage
It is used to detect PPRV antibody in serum of sheep and goat qualitatively, evaluate the immune status of Peste des petits ruminants vaccine and assisted serological diagnosis of infected animals.
2. Principle
This kit use competition ELISA method, PPRV antigen is pre-coated on enzyme micro-well strips. When testing, add serum sample and Monoclonal Enzyme conjugate, after incubation, if there is PPR virus specific antibody, it will bind to the PPRV antigen on the coating plate and prevent the enzyme-labeled monoclonal antibody from binding to the antigen on the plate; conversely, if the sample does not contain PPRV-specific antibody, it will not bind to the coating plate. After washing to remove the unbound antibody and other components, add substrate to the microwells to form a blue product through enzymatic catalysis. After adding stop solution to terminate the reaction, use a microplate reader at 450nm/630nm double wavelength to measure the absorbance A value in the reaction well.
3. The kit components
1 | PPRV antigen coated microplate | 96T X 2 | |
2 | Enzyme conjugate | 12ml | yellow lid |
3 | Sample dilution | 12ml | transparent lid |
4 | PPRV-IgG Negative control serum | 1.5ml | green lid |
5 | PPRV-IgG Positive control serum | 1.5ml | red lid |
6 | Substrate | 12ml X2 | orange lid |
7 | Stop solution | 12ml | blue lid |
8 | 10×concentrated washing buffer | 50ml | white lid |
9 | Adhesive Foil | 2 pieces | |
10 | Instruction | 1 piece | |
4. Material required not provided
1) Microplate Reader (double-wave length: 450/630 nm).
2) Precise micropipette (single-channel 10-100ul、0.5-10ul、multi-channel 30-300ul)
3) Constant temperature box or water bath box.
4) Oscillator.
5) Disposable tips (10ul, 200ul)
6) Deionized water
5.Storage and expire date
Store at 2~8℃ in dark, no frozen, expiry date: 12 months.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090 Fax 86-755-28938800 Email: info@lsybt.com www.lsybt.com
