Brucellosis antibody ELISA kit for bovine, goat, sheep, camels, pig and dog
- Sep 09, 2026 Anon
Brucellosis antibody ELISA kit for bovine, goat, sheep, camels, pig and dog
Catalog No. LSY-30036
1. Introduction
The Brucellosis antibody ELISA kit is used to test Brucellosis antibody in serum of bovine, goat, sheep, camels, pig and dog etc.
This kit use competitive ELISA method, the purified Brucella abortus antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample and Monoclonal antibody working solution, after incubation, if there is Brucellosis specific antibody in the sample, it will compete with antibody in Monoclonal antibody working solution to combine with the pre-coated antigen, discard the uncombined antibody and other components by washing; then add Enzyme Conjugate, the enzyme reacts with the monoclonal antibody that binds on the plate, discard the uncombined enzyme conjugate by washing; Add TMB substrate in micro-wells, the blue signal by Enzyme catalysis is in inverse proportion of antibody content in sample, use ELISA reader at 450nm wavelength to measure the absorbance OD value in reaction wells after adding stop solution to stop the reaction.
2. Reagents and contents
Code | Components | Specifications & Quantity |
192T | ||
1 | Brucellosis-Ag Coated plates 96wells | 2 plates |
2 | Enzyme Conjugate | 11ml *2 |
3 | 10X Concentrated washing buffer | 100ml *1 |
4 | Substrate | 22ml *1 |
5 | Sample diluent | 100ml *1 |
6 | Stop solution | 15ml *1 |
7 | Negative control | 500μl *1 |
8 | Positive control | 150μl *1 |
9 | Lyophilized monoclonal antibody | 6ml *2 |
10 | Adhesive Foil | 4 pieces |
11 | Instruction sheet | 1 piece |
3. Material required not provided
1) Micropipette: 0.5ul-10ul, 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells.
5) Distilled water or deionized water.
6) Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis
5. Storage and expire date
Store at 2~8℃ in dark, no frozen, expiry date: 12 months.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
