Mycobacterium tuberculosis(Mycobacterium Bovis) antibody ELISA test kit for bovine
- Sep 09, 2026 Anon
Mycobacterium tuberculosis(Mycobacterium Bovis) antibody ELISA test kit
Catalog No. LSY-30046
1. Introduction
The Mycobacterium tuberculosis(Mycobacterium Bovis) antibody ELISA kit is used to test Mycobacterium tuberculosis antibody in bovine serum. It is used to evaluate the serologic assistant diagnosis of tuberculosis caused by Mycobacterium bovis.
This kit use indirect ELISA method, Mycobacterium tuberculosis antigen is pre-coated on enzyme micro-well strips. When testing, add diluted serum sample, after incubation, if there is Mycobacterium tuberculosis specific antibody, it will combine with the pre-coated antigen, discard the uncombined antibody and other components with washing; then add enzyme labled anti-Mycobacterium tuberculosis antibody, it will combine with the complex of antibody and pre-coated antigen on plate; discard the uncombined enzyme conjugate with washing; Add TMB substrate in micro-wells, blue product formed enzymatically, use ELISA reader at 450nm wavelength to measure the absorbance A value in reaction wells after adding stop solution to stop the reaction.
2. Reagents and contents
Code | Item | Spec. | Code | Item | Spec. |
1 | Tuberculosis-Ag Coated plates 96wells | 2 plate | 7 | Stop solution | 15 ml |
2 | Enzyme Conjugate | 11 ml X2 | 8 | Negative control | 2 ml |
3 | 10X Concentrated washing buffer | 100 ml | 9 | Positive control | 1.6 ml |
4 | Substrate | 22 ml | 10 | Adhesive Foil | 4 pieces |
5 | Sample diluent | 100 ml | 11 | Instruction sheet | 1 piece |
6 | Serum dilution plate | 2 piece |
3. Material required not provided
1) Micropipette: 0.5µl-10µl, 10ul-100ul, 100ul-1000ul.
2) Disposable pipette tips.
3) Graduate: 500ml.
4) Microplate Reader: 96 wells.
5) Distilled water or deionized water.
6) Microplate Washer
4. Sample preparation
Take animal whole blood, get serum by using regular method, the serum should bright and no hemolysis.
5. Storage and expire date
Store at 2~8℃ in dark, no frozen, expiry date: 12 months.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
