Classical Swine Fever Virus antibody ELISA test Kit for pig
- Sep 09, 2026 Anon
Classical Swine Fever Virus antibody ELISA test Kit
Catalog No. LSY-30006
1. Usage
The GreenSpring® Classical Swine Fever Virus(CSFV) antibody ELISA Test kit is used for detection of Classical Swine Fever Virus antibody in porcine serum; assessment of immunity conditions against Classical Swine Fever Virus in the pig farm and investigation of the epidemiology of the Classical Swine Fever Virus.
2. Principle
The GreenSpring® Classical Swine Fever Virus(CSFV) antibody ELISA test kit is made from the antigen coated microtiter plate(coated with CSFV antigen) and other reagents. It applies the Solid-phase ELISA principle to CSFV-Ab in serum, then add enzyme conjugate to specifically bind with complex of coated antigen+CSFV-Ab+enzyme labeled anti-pig-IgG antibody on the microplate. With the TMB substrate, it will generate an amount of color. The depth of color is relative with the content of the CSFV-Ab, when the value of color is greater than the cut-off value, the pigs are vaccinated well or natural infected exist.
3. The kit components
1 | CSFV antigen coated microplate | 96T X 2 | |
2 | Enzyme conjugate | 22ml | yellow lid |
3 | Sample diluent solution | 50ml | transparent lid |
4 | CSFV-IgG Negative control serum | 1.5ml | green lid |
5 | CSFV-IgG Positive control serum | 1.5ml | red lid |
6 | Substrate | 12ml X2 | orange lid |
7 | Stop solution | 12ml | blue lid |
8 | 10×concentrated washing buffer | 50ml | white lid |
9 | Adhesive Foil | 2 pieces | |
10 | Instruction | 1 piece | |
4. Materials Required But Not Provided
1 Microplate Reader (double-wave length: 450/630 nm).
2 Precise micropipette (single-channel 1-100ul、0.5-10ul、multi-channel 30-300ul)
3 Constant temperature box or water bath box.
4 Oscillator.
5 Microplate Washer.
6 Disposable tips (10ul, 200ul)
7 Deionized water
5. Precautions and warnings for users
1. Do not use reagents expired, do not mix reagents from different lots.
2. Read the Manual carefully before use.
3. Experiment rubbish should be dealt with high pressure steam sterilization at 121 ℃ for 30 minutes, or treated with 5.0g/L sodium hypochlorite disinfectant for 30 minutes, then discard.
4. MicroWell plate removed from the refrigerated environment should be balanced moisture to dry at room temperature, then can be opened. Put back unused MicroWell plate into dry foil bag and sealed at 4 ℃. Unused liquid reagent should cover caps, store at 2-8 ℃ in dark with other group components.
5. Should use Micropipettor to add sample and reagents, and often proof its accuracy.
6. When adding washing buffer, should be full but no overflow, avoid appearing free enzyme at mouth of well or cross pollution between wells.
7. Stop solution is corrosive, use large amount of water to wash immediately when touch the skin or clothes.
Specifications: 96 wells×2.
Expiry date: 12 months.
Storage: Store at 2~8℃, in the dark.
Production Date: On outer-packing of the test kit.
For veterinary diagnosis only
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
