Porcine Pseudorabies gB Antibody ELISA Test Kit for pig
- Sep 09, 2026 Anon
Porcine Pseudorabies gB Antibody ELISA Test Kit
Catalog No. LSY-30004
1. Usage
The GreenSpring® Porcine Pseudorabies (PRV) Antibody ELISA Test Kit is used for detection of porcine Pseudorabies gB antibody in porcine serum; assessment of immunity conditions against porcine Pseudorabies in the pig farm and investigation of the epidemiology of the porcine Pseudorabies. While the result cannot distinguish immune antibody and infectious antibody, to confirm that is antibody by vaccine or wild virus infection needs to combine clinical data, immune status, and other diagnostic methods.
2. Principle
The GreenSpring® Porcine Pseudorabies (PRV) gB antibody ELISA test kit uses the principle of solid-phase enzyme-linked immunosorbent assay (ELISA), and consists of a microwell reaction plate coated with PRV gB VP1 structural protein antigen, anti-pig IgG labeled with horseradish peroxidase, and other reagents. The reaction mechanism is that the coated antigen is combined with the PRV-gB-VP1-Ab in the sample, and then combined with the enzyme-labeled anti-pig IgG antibody to form a "coated antigen+PRV-gB-VP1-Ab+enzyme-labeled anti-pig IgG antibody" complex. Add adding Substrate, Catalytic reaction color development. The color depth is directly proportional to the amount of PRV-gB-VP1-Ab . When the color reaction of the sample is detected, the result obtained by the microplate reader exceeds the set threshold, and the result is positive, which indicates that the pigs are vaccinated well or natural infected exist.
3. The kit components
1 | PRV-gB antigen coated microplate | 96T X 2 | |
2 | Enzyme conjugate | 22ml | yellow lid |
3 | Sample diluent solution | 50ml | transparent lid |
4 | PRV-gB Negative control serum | 1.5ml | green lid |
5 | PRV-gB Positive control serum | 1.5ml | red lid |
6 | Substrate | 12ml X2 | orange lid |
7 | Stop solution | 12ml | blue lid |
8 | 10×concentrated washing buffer | 50ml | white lid |
9 | Adhesive Foil | 2 pieces | |
10 | Instruction | 1 piece | |
4. Materials Required But Not Provided
1 Microplate Reader (double-wave length: 450/630 nm).
2 Precise micropipette (single-channel 1-100ul、0.5-10ul、multi-channel 30-300ul)
3 Constant temperature box or water bath box.
4 Oscillator.
5 Disposable tips (10ul, 200ul)
6 Deionized water
5. Precautions and warnings for users
1. Read the Manual carefully before use.
2. Do not use reagents expired, do not mix reagents from different lots.
3. Experiment rubbish should be dealt with high pressure steam sterilization at 121 ℃ for 30 minutes, or treated with 5.0g/L sodium hypochlorite disinfectant for 30 minutes, then discard.
4. MicroWell plate removed from the refrigerated environment should be balanced moisture to dry at room temperature, then can be opened. Put back unused MicroWell plate into dry foil bag and sealed at 4 ℃. Unused liquid reagent should cover caps, store at 2-8 ℃ in dark with other group components.
5. Should use Micropipettor to add sample and reagents, and often proof its accuracy.
6. When adding washing buffer, should be full but no overflow, avoid appearing free enzyme at mouth of well or cross pollution between wells.
7. Stop solution is corrosive, use large amount of water to wash immediately when touch the skin or clothes.
Specifications: 96 wells×2.
Expiry date: 12 months.
Storage: Store at 2~8℃, in the dark, no frozen.
Production Date: On outer-packing of the test kit.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
