Porcine Foot-and-Mouth Disease Virus 3ABC Antibody ELISA Test Kit for swine
- Sep 10, 2026 Anon
Porcine Foot-and-Mouth Disease Virus 3ABC Antibody ELISA Test Kit
Catalog No. LSY-30003
1. Usage
The GreenSpring® Porcine Foot-and-Mouth Disease Virus 3ABC ELISA Test Kit is used for detection of FMD non-structural 3ABC-IgG antibody in porcine serum qualitatively; it is used to distinguish Porcine FMD antibody between wild virus infection and produced by inactivated vaccine.
2. Principle
The GreenSpring® Porcine Foot-and-Mouth Disease Virus 3ABC ELISA Test Kit is made from the antigen coated microtiter plate(coated with 3ABC antigen) and other reagents. It applies the Solid-phase ELISA principle to FMD-3ABC-Ab in serum, then add enzyme conjugate to specifically bind with complex of coated antigen+FMD-3ABC-Ab+enzyme labeled anti-pig-IgG antibody on the microplate. With the TMB substrate, it will generate an amount of color. Read the OD value with ELISA reader at 450nm/630nm after stop reaction, the OD value will reflect the level of 3ABC antibody in porcine serum.
3. The kit components
1 | FMD-3ABC antigen coated microplate | 96T X 2 | |
2 | Enzyme conjugate | 22ml | yellow lid |
3 | Sample diluent solution | 50ml | transparent lid |
4 | 3ABC-IgG Negative control serum | 1.5ml | green lid |
5 | 3ABC-IgG Positive control serum | 1.5ml | red lid |
6 | Substrate | 12mlX2 | orange lid |
7 | Stop solution | 12ml | blue lid |
8 | 20×concentrated washing buffer | 50ml | white lid |
9 | Adhesive Foil | 6 pieces | |
10 | Instruction | 1 piece | |
Note: do not mix use reagents from different lot no.
4. Materials Required But Not Provided
1 Microplate Reader (double-wave length: 450/630 nm).
2 Precise micropipette (single-channel 1-100ul、0.5-10ul、multi-channel 30-300ul)
3 Constant temperature box or water bath box.
4 Oscillator.
5 Microplate Washer.
6 Disposable tips (10ul, 200ul)
7 Deionized water
5. Precautions and warnings for users
1. This test kit is for research use only.
2. Wear glove and working clothes when operate, treat the test kit as containing infectious material.
3. Experiment rubbish should be dealt with high pressure steam sterilization at 121 ℃ for 30 minutes, or treated with 5.0g/L sodium hypochlorite disinfectant for 30 minutes, then discard.
4. MicroWell plate removed from the refrigerated environment should be balanced moisture to dry at room temperature, then can be opened. Put back unused MicroWell plate into dry foil bag and sealed at 4 ℃. Unused liquid reagent should cover caps, store at 2-8 ℃ in dark with other group components.
5. If the 20×concentrated washing buffer appears crystal, it is normal, put at 37℃ until been dissolved.
6. Should use Micropipettor to add sample and reagents, and often proof its accuracy.
7. When adding washing buffer, should be full but no overflow, avoid appearing free enzyme at mouth of well or cross pollution between wells.
8. Stop solution is corrosive, use large amount of water to wash immediately when touch the skin or clothes.
Specifications: 96 wells×2.
Expiry date: 12 months.
Storage: Store at 2~8℃, in the dark.
Production Date: On outer-packing of the test kit.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
