Porcine Foot and Mouth Disease Virus (FMDV) Type A antibody ELISA Kit for swine
- Sep 10, 2026 Anon
Porcine Foot and Mouth Disease Virus (FMDV) Type A antibody ELISA Kit
Catalog No. LSY-30037
1. Usage
The GreenSpring® Porcine FMDV Type A antibody ELISA Test kit is used for detection of Porcine Foot And Mouth Disease Type A antibody in porcine serum; assessment of immunity conditions against porcine FMD in the pig farm and investigation of the epidemiology of the porcine FMD.
2. Principle
This test kit is based on the principle of solid-phase enzyme-linked immunosorbent assay (ELISA), consisting of microwell reaction plate coated with VP1 structural protein of pig FMD Type A, horseradish peroxidase-labeled anti-pig IgG and other reagents. The reaction mechanism is coated antigen combine with FMDV-A-VP1-Ab in the sample, then combine with enzyme labled anti-pig IgG to form a complex of “coated antigen+FMDV-A-VP1-Ab+ enzyme-labeled anti-pig IgG antibody”, add substrate, color appear by enzyme catalysis reaction. The color depth is directly proportional to the amount of FMDV-A-VP1-Ab. When the sample reaction is chromogenic, the result is positive when the results measured by ELISA reader exceed the set threshold value, which means that immunity has produced antibodies or natural infection exist.
3. The kit components
1 | FMD-A antigen coated microplate | 96T X 2 | |
2 | Enzyme conjugate | 22ml | yellow lid |
3 | Sample diluent | 50ml | transparent lid |
4 | FMD-A-IgG Negative control serum | 1.5ml | green lid |
5 | FMD-A-IgG Positive control serum | 1.5ml | red lid |
6 | Substrate | 12ml X2 | orange lid |
7 | Stop solution | 12ml | blue lid |
8 | 20×concentrated washing buffer | 50ml | white lid |
9 | Adhesive Foil | 2 pieces | |
10 | Instruction | 1 piece | |
4. Materials Required But Not Provided
1) Microplate Reader (double-wave length: 450/630 nm).
2) Precise micropipette (single-channel 1-100ul、0.5-10ul、multi-channel 30-300ul)
3) Constant temperature box or water bath box.
4) Oscillator.
5) Disposable tips (10ul, 200ul)
6) Deionized water
5. Precautions and warnings for users
1) This test kit is for research use only.
2) Do not use reagents expired, do not mix reagents from different lots.
3) Read the Manual carefully before use.
4) Experiment rubbish should be dealt with high pressure steam sterilization at 121 ℃ for 30 minutes, or treated with 5.0g/L sodium hypochlorite disinfectant for 30 minutes, then discard.
5) MicroWell plate removed from the refrigerated environment should be balanced moisture to dry at room temperature, then can be opened. Put back unused MicroWell plate into dry foil bag and sealed at 4 ℃. Unused liquid reagent should cover caps, store at 2-8 ℃ in dark with other group components.
6) Should use Micropipettor to add sample and reagents, and often proof its accuracy.
7) When adding washing buffer, should be full but no overflow, avoid appearing free enzyme at mouth of well or cross pollution between wells.
8) Stop solution is corrosive, use large amount of water to wash immediately when touch the skin or clothes.
Specifications: 96 wells×2.
Expiry date: 12 months.
Storage: Store at 2~8℃, in the dark, no freezing.
Production Date: On outer-packing of the test kit.
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
