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Nucleic Acid Detection Kit for Bacillus anthracis (Fluorescent PCR Method)

• Oct 26, 2026   Anon

Nucleic Acid Detection Kit for Bacillus anthracis (Fluorescent PCR Method)

Catalog Number: PA51A/01

Version Number: Version 1.0

Packing: 50T/kit

Product description:

Utilizing TaqMan probe technology, specific primers and probes are designed targeting conserved genes of *Bacillus anthracis* to facilitate the auxiliary detection of *Bacillus anthracis* nucleic acids in samples. This product incorporates a dUTP/UDG system capable of degrading ssDNA and dsDNA containing uracil (U), thereby eliminating cross-contamination caused by PCR products.

Product Composition:

Note: Products from different batches must not be mixed or substituted for one another!!!

Storage and Shelf Life:

Store at-20 °C, protected from light; shelf life: 12 months. Transport using dry ice. Limit repeated freeze-thaw cycles to fewer than 5 times.

Applicable instruments:

ABI 7500, SLAN®-96P, Bio-Rad CFX96 real-time quantitative PCR instruments, etc.

Sample Requirements:

1. Applicable sample types

Samples including suspected contaminated soil, dust or object surfaces; wipes for animal fur are placed in 50% glycerol physiological saline solution; suspected anthrax skin ulcer lesion secretion in livestock; blood, cerebrospinal fluid, phlegm,vomit or feces.

Fresh samples must be submitted for testing; repeated freeze-thaw cycles are prohibited!

2. Sample Processing (Nucleic Acid Extraction Area)

Perform sample pre-treatment in accordance with standards such as "NY/T 541-2016 Technical Specifications for Sampling, Preservation, and Transportation of Veterinary Diagnostic Samples," and use commercial nucleic acid extraction kits to extract nucleic acids from the samples. The extracted nucleic acids shall be analyzed immediately; otherwise, they shall be stored at

-20 °C for no more than 6 months, and repeated freeze-thaw cycles are strictly prohibited!

Result Assessment:

1. When the FAM channel Ct value is ≤35 or VIC channel Ct value is ≤35, one of them match, the sample is judged to be positive.

2. When the FAM channel Ct value or VIC channel Ct value falls within the range of 35 <Ct <40, it is necessary to observe whether the amplification curve of the target gene exhibits an S-shape. If the curve is S-shaped, the sample should be regarded as a suspected positive, and re-testing is required; if the re-test result shows a Ct value ≤ 40 and displays a typical S-shaped curve, it shall be classified as positive; otherwise, if no Ct value is detected, the Ct value >40, or the curve does not exhibit a typical S-shape, the sample shall be classified as negative.

3. If the FAM channel Ct value and VIC channel Ct value is  > 40 or no Ct value is displayed, the test sample is judged to be negative.

Limitations of the testing method:

1. A negative result may be attributed to factors such as low-quality nucleic acids extracted from the sample, inappropriate storage conditions, an unsuitable storage duration, the presence of inhibitors in the sample, nucleic acid degradation, or poor extraction efficiency of the nucleic acid extraction protocol.

2. Unreasonable sample collection, transportation, and storage conditions; an insufficient viral load in the sample; or inappropriate laboratory procedures and environmental conditions may all lead to false-negative or false-positive results. A comprehensive assessment should be conducted by integrating other clinical symptoms and relevant data, with repeat testing performed when necessary.

3. Due to mutations or other factors, sequence variations in the target sequence may lead to false-negative results.

Manufacturer: Shenzhen Lushiyuan Biotechnology Co., Ltd.

Address: D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120, China.

Tel. 86-755-28438788/86-18165709090

Email: info@lsybt.cm 

Website: www.lsybt.com