Bovine Brucellosis Indirect ELISA Antibody Test Kit
- Oct 26, 2026 Anon
Bovine Brucellosis Indirect ELISA Antibody Test Kit
Catalog No.: LSY-30036B
[ Components and Contents]
Code | Kit Components | Quantity |
1 | Brucellosis antigen-coated plate | 96 wells/plate × 2 plates |
2 | Sample diluent | 50 ml/bottle × 1 bottle |
3 | Negative control | 1.5 ml/ vial × 1 vial |
4 | Positive control | 1.5 ml/ vial × 1 vial |
5 | 10×concentrated washing buffer | 50 ml/bottle × 1 bottle |
6 | Enzyme conjugate | 22 ml/bottle × 1 bottle |
7 | Substrate | 12 ml/bottle × 2 bottles |
8 | Stop solution | 12 ml/bottle × 1 bottle |
9 | Adhesive Foil | 2 sheets |
10 | Instructions | 1 copy |
[Function and Purpose]
Brucellosis is a zoonotic infectious disease caused by Brucella bacteria. It is characterized by inflammation, necrosis, and the formation of granulomas in the reproductive organs, fetal membranes, and various other organ tissues, resulting in symptoms such as miscarriage, infertility, orchitis, and arthritis. A variety of animals exhibit varying degrees of susceptibility to the disease; natural infections are most common in sheep, cattle, and pigs. Animal infection can occur through multiple routes, including the digestive tract, the skin and mucous membranes, and vertical transmission. Brucellosis poses a serious threat to human health and adversely affects the development of animal husbandry in the worldwide.
The microwell plates in this kit are coated with purified Brucella antigens; the indirect ELISA method is used to detect Brucella antibodies in bovine serum. The control and the diluted serum sample are added to the wells; if Brucella antibodies are present in the sample, an antigen–antibody complex will form. After washing the plate, horseradish peroxidase (HRP)-labeled antibodies are added to the wells; the enzyme conjugate binds to the Brucella antibodies, forming an "antigen–antibody–enzyme conjugate" complex. After washing the plate to remove excess enzyme conjugate, a substrate solution (TMB) is added, which is enzymatically catalyzed to produce a blue product. The color development depends on the amount of specific antibodies present in the test sample: if antibodies are present, a blue color develops; after the addition of the stop solution, the color changes to yellow; if no antibodies are present, no color reaction occurs. The absorbance (OD) of the reaction plate is measured using a microplate reader at a wavelength of 450 nm.
[Storage and expire date]
Store at 2–8℃; shelf life: 12 months.
[Specifications]
96*2wells/kit (2plates/kit)
For Veterinary Use Only
Shenzhen Lvshiyuan Biotechnology Co., Ltd
D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120 China
Tel. 86-755-28438788/86-18165709090
Fax 86-755-28938800
Email: info@lsybt.com
www.lsybt.com
