Foot-and-Mouth Disease Virus-Universal (FMDV-U) nucleic acid test kit
- 2026-10-26 佚名
Foot-and-Mouth Disease Virus-Universal (FMDV-U) nucleic acid test kit
(Fluorescent RT-PCR)
Catalog No. | Specifications | Storage | Transport | Version number |
PA30A/1 | 50 T | -20℃ | low temperature | V1 |
[Product Overview]
This product is a one-step qRT-PCR nucleic acid test kit designed for the auxiliary detection of FMDV nucleic acid in samples. Specific primers and a specific fluorescent probe targeting the conserved region of the FMDV 3D gene have been developed; the probe is labeled with the FAM fluorescent dye. When combined with the qRT-PCR reaction mix, this kit enables rapid detection of FMDV-U nucleic acid using a fluorescent quantitative PCR instrument.
The kit includes a positive control and a negative control. The positive control consists of a plasmid containing the amplification fragment, used to assess the validity of the assay; the negative control is used to detect the presence of contamination in the reaction system.
[Product Composition]
Code | Component | PA30A�1 |
1 | One-step qRT-PCR enzyme mix | 80 μL |
2 | FMDV-U qRT-PCR amplification reaction mix | 1250 μL |
3 | Positive control | 100 μL |
4 | Negative control | 100 μL |
[Storage Conditions]
Store at-20 °C, protected from light; shelf life: 12 months.
[Applicable Instruments]
Real-time fluorescent PCR instruments, such as the ABI 7500, Bio-Rad CFX96, and SLAN®-96P.
[Sample Requirements]
1. Customers are required to provide their own reagents: normal saline.
2. Collected serum, blister skin, blister fluid and other sample should be stored and transported at 2–8 °C and promptly submitted for laboratory testing. Alternatively, the samples may be preserved in viral transport medium and then sent to the laboratory for analysis.
Note: Samples submitted for testing must be fresh; repeated freeze-thaw cycles are strictly prohibited.
3. It is recommended to use a commercial viral nucleic acid extraction kit for RNA extraction and purification; the extracted RNA can then be directly used for detection. If samples are not analyzed immediately after extraction, they may be stored at-80 °C for later use; however, repeated freeze-thaw cycles should be avoided.
[Interpretation of result]
1. Experimental setup requirements
The reported fluorescence for FMDV-U in this product is FAM.
1). Positive control: The FAM channel exhibited a characteristic S-shaped amplification curve, and all Ct values were ≤35;
2). Negative control: No Ct value detected in the FAM channel;
The above requirements must be met simultaneously within the same experiment; otherwise, the experiment will be invalidated, and it must be repeated.
2. Judgment criteria
Gene Name and Markers | 3Dgen(FAM) | Result Evaluation |
Results | Ct value ≤ 40, with evident exponential growth | FMDV-U positive |
When 40<Ct value ≤ 45, there is a pronounced exponential increase. | Suspicious result a | |
No Ct value | FMDV-U negative |
For suspicious results, a retest is required; if the Ct value in the retest is ≤45, the result is classified as positive; if no Ct value is obtained, the result is classified as negative.
Manufacturer: Shenzhen Lushiyuan Biotechnology Co., Ltd.
Address: D Building, National Biological Industrial Park of Marinelife, No.2 Binhai Road, Dapeng, Shenzhen, 518120, China.
Tel. 86-755-28438788/86-18165709090
Email: info@lsybt.cm
Website: www.lsybt.com